Glycerol tributylate promoted the liver lipid metabolism by cultivating the intestinal flora of Grass carp

Abstract

Abstract To investigate the effects of glycerol tributyrin (TB) on the regulation of liver lipid metabolism by intestinal flora of grass carp. The compound feed with soybean oil 2.8% + fish oil 1.8%, soybean oil 6.3% + fish oil 1.8% and soybean oil 6.2% + fish oil 1.8% + TB 0.1% were added to the basal diet as a fat source and fed to the basal (Control) group, high lipid (HL) group, and tributyrin (TB) group, respectively. After 12 weeks, 16S rRNA sequencing was conducted on the intestinal flora of grass carp. The results found that the grass carp intestinal microflora in the TB group was more abundant and complex than in the HL group, with a number of novel and unique microflora. To summarize, a high-fat diet can induce the development of nutritional fatty liver in the host by affecting the composition of the intestinal microbial community, and TB can exert its lipid-lowering effects under high-fat diet conditions by affecting the composition of the intestinal microbial community.