Affiliation:
1. Tianjin Medical University
2. Tianjin Huanhu Hospital
Abstract
Abstract
Background: Long noncodingRNAs (lncRNAs) are considered key players in the formation and development of tumors.Herein, Gene Expression Profiling Interactive Analysis (GEPIA) was employed as a bioinformatics technology. LINC02587 is differentially expressed in bladder urothelial cancer, glioblastoma, lung adenocarcinoma, lung SCC,melanoma,and other tumortissue and cells. However, its impacton the emergenceof glioma and its mechanism is remaining elusive.
Methods: Some of the in vitro assays employed in this study were the CCK-8 / Annexin-V / Transwell assays, colonyformation, and wound healing, together with Western blot (WB) evaluation. MSP / BSP assays were employed for assessing the CpG island'smethylation status in the LINC02587 promoter. Through transcriptome, ferroptosis-related experiments, and WB evaluation, it was confirmed that LINC02587 is correlated with the regulation of ferroptosisin tumor cells, and CoQ-Fsp1 is one of its regulatory pathways. Moreover, the underlined in-vitro results were further validated by in-vivo studies.
Results: The current study shows that the promoter sequenceof LINC02587 is regulated by methylation. The silencing of LINC02587 can inhibit cellular proliferative, migrative, and invasive properties, and induce ferroptosis within gliomas through the CoQ-FSP1 pathway.
Conclusions: LINC02587 is likelyto be a novel drug target in treating glioma.
Publisher
Research Square Platform LLC
Reference29 articles.
1. CBTRUS Statistical Report: primary brain and other central nervous system tumors diagnosed in the United States in 2010–2014;Ostrom QT,2017
2. Glioma tumor grade identification using artificial intelligent techniques;Ahammed Muneer KV;J. Med. Syst.,2019
3. Olson,Exciting new advances in neuro-oncology: the avenue to a cure for malignant glioma;Meir EG;CA Cancer J. Clin.,2010
4. On the classification of long non-coding RNAs;Ma L;RNA Biol,2013
5. Long noncoding RNA and cancer: a new paradigm;Bhan A;Cancer Res,2017