Affiliation:
1. General Hospital of Northern Theater Command
2. Third Affiliated Hospital of Second Military Medical University
Abstract
Abstract
Glioblastomas (GBM) are most common types of primary brain tumors and miRNAs play an important role in pathogenesis of glioblastomas. Here, we reported a new miRNA, miR-3154, which regulates glioblastoma proliferation and metastasis. miR-3154 was elevated in glioblastoma tissue and cell lines, and its elevation was associated with grade of glioblastomas. Knockdown of miR-3154 in cell lines weakened ability of proliferation and colony formation, and caused cell cycle arrested and higher percentage of apoptosis. Knockdown of miR-3154 also impaired ability of migration and invasion in glioblastoma cells. In mechanism, miR-3154 bound directly to Tumor Protein P53 Inducible Nuclear Protein 1 (TP53INP1), down-regulating TP53INP1 expression at both mRNA and protein level. Silence of TP53INP1 reversed the effect of miR-3154 knockdown on proliferation and metastasis of glioblastoma cells. These findings show that miR-3154 promotes glioblastoma proliferation and metastasis via targeting TP53INP1.
Publisher
Research Square Platform LLC
Reference28 articles.
1. Weller M, Wick W, Aldape K, Brada M, Berger M, Pfister SM, Nishikawa R, Rosenthal M, Wen PY, Stupp R, et al: Glioblastoma. Nature reviews Disease primers 2015, 1:15017.
2. European Association for Neuro-Oncology (EANO) guideline on the diagnosis and treatment of adult astrocytic and oligodendroglial glioblastomas;Weller M;Lancet Oncol,2017
3. Advances in the molecular genetics of glioblastomas - implications for classification and therapy;Reifenberger G;Nat reviews Clin Oncol,2017
4. Current state of immunotherapy for glioblastoma;Lim M;Nat reviews Clin Oncol,2018
5. EANO guideline for the diagnosis and treatment of anaplastic glioblastomas and glioblastoma;Weller M;Lancet Oncol,2014