CEP55 leads to poor prognosis of glioma by regulating the stemness and immune microenvironment

Abstract

Abstract High recurrence rate of glioma makes it difficult to treat. The tumor microenvironment- and the presence of stem cells- in glioma are believed to be important factors in regulating its recurrence. The role of centrosomal protein 55 (CEP55) in promoting mitosis and regulating tumorigenesis in various cancers has been previously explored. We comprehensively sought to reveal the regulatory role of CEP55 in glioma.We analyzed the data from the cancer genome atlas (TCGA) and Chinese glioma genome atlas (CGGA) database by bioinformatic and validated it in glioma-initiating cells (GIC) as well as in animal model. The overexpression of CEP55 in glioma is associated with the poor prognosis. The GO and KEGG analysis of the module most related to CEP55 from the WGCNA results were found to be enriched in genes associated DNA replication and cell cycle. The second most significant module were enriched in genes associated with extracellular matrix (ECM) organization and ECM receptor interaction. The immune infiltration analysis showed that the overexpression of CEP55 is associated with the levels of higher immune infiltration and immune checkpoint genes allowing accurate identification of glioma subtypes.Our study is a preliminary demonstration of the multifaceted role of CEP55 in glioma regulation. CEP55 promotes the malignant progression of glioma by both regulating glioma stem cells and the immune microenvironment thus, providing a new perspective for the diagnosis and treatment of glioma.