Restoration of miR-200c sensitizes breast cancer cells to cytarabine

Abstract

Abstract Background Breast cancer is one of the most common cancers affecting women, which has led researchers to explore novel treatment strategies. A crucial obstacle in the treatment of breast cancer is the development of acquired chemoresistance. miR-200c has been found to be associated with decreased chemoresistance in various types of cancer. Mehtods and results: The results of the MTT assay indicated that the restoration of miR-200c in combination with cytarabine sensitized MDA-MB-468 cells to cytarabine, resulting in the inhibition of cell proliferation. Moreover, its restoration led to cell cycle arrest in the sub-G1 phase. Furthermore, qRT-PCR demonstrated reduced levels of c-Myc and Cyclin D1, as well as increased levels of p21. Our findings indicate that miR-200c increases the sensitivity of MDA-MB-468 cells to cytarabine, regulates cell cycle-related genes, and inhibits the growth of MDA-MB-468 cells. Conclusion Our results suggest that the restoration of miR-200c, in combination with cytarabine, could be a promising and innovative approach to treat breast cancer.