Affiliation:
1. National Institute of Nutrition and Food Safety: Chinese Center for Disease Control and Prevention National Institute for Nutrition and Health
2. Chinese Academy of Preventive Medicine Institute of Nutrition and Food Hygiene: Chinese Center for Disease Control and Prevention National Institute for Nutrition and Health
Abstract
Abstract
Purpose
We aimed to investigate the effect of tatABC gene deletion on the pathogenicity of enterotoxigenic Escherichia Coli (ETEC).
Methods
Changes in bacterial dynamics and virulence after deletion were examined using mobility and animal toxicity tests for ETEC. Further, differential expression of proteins between the gene-deletion and wild-type strains was analyzed using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to explore the possible mechanisms underlying the effects of this gene on bacterial virulence.
Result
After tatABC knockout, the virulence of ETEC H10407 was strongly attenuated, and its invasion in rabbit intestinal mucosa was significantly reduced. Mass spectrometry revealed that 10 differentially expressed proteins involved in physiological functions of bacteria, which may be associated with changes in bacterial motility and pathogenicity.
Conclusion
The research shows that the twin-arginine translocation system of ETEC may be closely related to its virulence.
Publisher
Research Square Platform LLC