In Vitro Cytotoxic Activity of Quercetin and Silymarin and Their Synergistic Effect on Hep G2 Cell Lines

Abstract

Abstract Purpose One of the biggest causes of mortality among people is cancer. Plants may contain potential bioactive substances that might be used to provide "new leads" in the fight against cancer and other disorders. The main objective of this present study was to evaluate the cytotoxic activities of Quercetin and Silymarin and their Synergistic effect on Hep G2. Method The cytotoxic activity of this individual drug and in combination was confirmed by the consideration of IC50 by MTT assay. Results The results show that IC50 of Quercetin 50 µg, Silymarin 40 µg, and combination 30 µg respectively. The combination of these drugs shows a significant increase in cytotoxic effect and anti-proliferative effect compared with individual drugs. Thus, the results indicate that this combination has anti -hyper carcinogenic effect.