Green Synthesis of SiO 2 NPs Promote Vigna radiata L Germination, Membrane Damage, Antioxidant enzymes and Physiological activities

Abstract

Abstract In agriculture sector demand for nanotechnology-based products in current years, have led to the increasing use of nanoparticles (NPs). Biologically prepared NPs have gained a lot of attraction due to ecofriendly, less expensive and biocompatible process, therefore, Current work was design to investigate the green synthesized SiO2 NPs on Vigna radiata L. physiological and biochemical analysis. Six days old plants (two leaf stage) exposed to SiO2 NPs at 2, 20, 200 and 2000 ppm. Significantly increased in plant length and weight at on all concentrations at different intervals except 20 ppm of SiO2 NPs reduced plant length and weight. However, the antioxidant enzymes at 20 ppm treated plant showed several effects. The production of ascorbate peroxidase and catalase increased as compared to control. Peroxidase level was initially increased but then reduced later, superoxide dismutase also reduced as compared to control. FT-IR at 20 ppm treated plant exhibited the increase in protein, decrease in phenolics and Bromo also increase which means alkaloid will produce to overcome the stress. Evans blue staining and membrane damage quantification also indicated the cell death at 20 ppm as compared to control at all time intervals. These results suggest that SiO2 NPs exhibit positive effects at higher concentrations and slightly reduce the growth at (20 ppm) mild concentrations. Si uptake and antioxidant enzymes also decrease at 20 ppm, Evans blue staining and quantification indicated about the slight damage.