Culture-dependent bacterial diversity of an invasive Bondar’s nesting whitefly, Paraleyrodes bondari Peracchi on different hosts

Abstract

Abstract The nymphs and adults of Paraleyrodes bondari collected on seven hosts from different locations of Karnataka, India during 2021–2023. 91 facultative bacterial colonies were isolated from nymphs and adults of P. bondari by using spread-plate technique and identified through 16srRNA sequencing. In which nymphs showed high bacterial abundance (54.95%) than adults (45.05%). The P. bondari collected on coconut harboured more (30) number of bacteria followed by banana (21). Phylum Bacillota was dominant (56.52%) in P. bondari followed by Pseudomonadota (30.43%). Bacilli was found dominant (43.33 to 72.73%) in P. bondari followed by Gamma-proteobacteria (10 to 38.10%). Among 13 orders, Bacillales was dominant (36 to 72%) followed by Enterobacteriales (9 to 20%). 23 to 60% of the bacteria were belong to Bacillaceae followed by Staphylococcaceae (4.76 to16.66%). Bacillus was dominant genus (23.33 to 60%) and B. cereus, B. licheniformis, B. subtilis were common in both nymphs and adults.