Affiliation:
1. Xiangya Hospital Central South University
2. Brain Hospital of Hunan Province
Abstract
Abstract
Coronary microvascular dysfunction (CMD) is an important pathogenesis of various cardiovascular diseases. Lower endothelial nitric oxide synthase (eNOS) phosphorylation leads to reduced endothelium-derived relaxing factor nitric oxide (NO) generation, causing and accelerating CMD. Endoplasmic reticulum stress (ER stress) has been shown to reduce NO production in umbilical vein endothelial cells. Oxidized low-density lipoprotein (ox-LDL) damages endothelial cell function. But the relationship between ox-LDL and coronary microcirculation has not been assessed. Short-chain fatty acid (SCFA) is fermentation products of the gut microbiome, could improve endothelial-dependent vasodilation in human adipose arterioles, the effect of SCFA on coronary microcirculation is unclear. In this study, we found ox-LDL stimulated expression of ER chaperone GRP78, and further activated downstream PERK/eIF2a, IRE1/ JNK and ATF6 signaling pathways, resulting in a decrease in eNOS phosphorylation and NO production in human cardiac microvascular endothelial. Furthermore, SCFA-propionate can inhibit ox-LDL-induced eNOS phosphorylation reduction, raise NO production, the mechanism is related to the inhibition of ER stress and downstream signaling pathways PERK/eIF2a, IRE1/JNK, and ATF6. In summary, we demonstrate that ox-LDL induced CMD by activating ER stress, propionate can effectively counteract the adverse effects of ox-LDL and protect coronary microcirculation function via inhibiting ER stress.
Publisher
Research Square Platform LLC