Restoration of gut dysbiosis through Clostridium butyricum and magnesium possibly balance blood glucose levels: an experimental study

Abstract

Abstract Diabetes mellitus (DM) is a chronic metabolic disorder characterized by an elevated level of blood glucose due to the absence of insulin secretion, ineffectiveness, or lack of uptake of secreted insulin in the body. The improperly diagnosed and poorly managed DM can cause severe damage to organs in the body like the nerves, eyes, heart, and kidneys. This study was aimed at investigating the effect of probiotic Clostridium butyricum with magnesium supplementation to evaluate the effect on gut microbial dysbiosis and blood glucose levels. In an animal house, 24 male albino rats weighing 200–250 g have free access to water and food. Diabetes was induced using streptozotocin 60 mg/kg in overnight fasted rats. Diabetic rats were divided into four groups (n = 6). Control group (G0) rats were given Metformin 100 mg/kg/day with a standard basal diet, treatment group 1 was given Clostridium Butyricum 2.5x105 CFU/day with standard basal diet, magnesium 500 mg/kg/day to group 2, While Clostridium Butyricum (1.5x105 CFU/day) and magnesium (300 mg/kg/day) in combination with a standard basal diet to group 3. Random blood glucose levels were monitored twice a week for 21 days and were represented as mean of each week. The results conclude that Clostridium Butyricum 2.5 x105 CFU is very effective in balancing random blood glucose levels from 206.6 ± 67.7 to 85.1 ± 3.8 (p = 0.006) compared to other groups (p > 0.005). The results of stool analysis showed that Clostridium Butyricum restores microbial dysbiosis as evident by the 105 CFU Clostridium Butyricum load in G1, which was higher than G0, G2 and G3 which were 103 and 104 CFU respectively. The findings of this study conclude that Clostridium Butyricum supplementation improved blood glucose levels and intestinal bacterial load in type II diabetes mellitus.