Affiliation:
1. Rajasthan University of Veterinary and Animal Sciences College of Veterinary and Animal Sciences: College of Veterinary and Animal Science Bikaner
2. College of veterinary and animal Science Navania
3. Rajasthan University of Veterinary and Animal Sciences
Abstract
Abstract
Background and Objectives: The present study has determined the serological diversity, virulence gene and antibiotic resistance gene profile, and in-vitro antibiogram of non-typhoidal Salmonella (NTS) from poultry enteritis in semiarid area of Rajasthan, India
Methods: Organized poultry farms, from six districts of semiarid regions of Rajasthan were tested for presence of Salmonella serovars. Thirty Salmonella enterica strains, were screened for the presence of 15 virulence genes by PCR. Further, resistance to eight antibiotics was tested phenotypically by Kirby–Bauer method and genotypically by PCR.
Results: NTS were isolated from 78.17%( N= 178/151) samples. These were serologically belonged to . S. Typhimurium (51 strains), S. Weltevreden (90 strains) and untypable (10 strains). Out of these, Thirty Salmonella strains (S. Weltevreden, ten strains, Salmonella Typhimurium ten strains and two untypable strains) were subjected to virulence gene profiling and phenotypic tests. Eleven virulence genes i.e. invA, hilA, sivH, tolC, agfA, lpfA, spaN, pagC, spiA, iroN and fliC genes were present in all the 30 isolates. While, sopE was present in only 2 isolates but spvC, cdtB and sefA were not detected in any of the isolates. Antibiotic sensitivity test revealed multidrug resistance in 70% (N=21/30) of the Salmonella isolates. All twenty-one multidrug resistant Salmonella stains were tested for presence of antimicrobial resistant genes i.e. blaTEM, blaSHV, blaCTX-M, tet(A), tet(B), tet(C), sul1, sul2, sul3. blaTEM and tet(A) which were present in 95% (20/21) isolates and sul2 gene was present in all isolates.
Conclusion: NTS strains with characteristics of pathogenicity and multidrug resistance from poultry enteritis were detected. These virulent salmonella strains may be responsible for causing poultry enteritis. These NTS strains have zoonotic implications and multidrug resistance showed necessity of prudent use of antibiotics in poultry industry.
Publisher
Research Square Platform LLC
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