Affiliation:
1. Padjadjaran University / Dr. Hasan Sadikin General Hospital
2. Maranatha Christian University
Abstract
Abstract
Purpose: This study aimed to analyze the differences and correlation of progesterone receptor-B (PR-B) promoter methylation and mRNA DNA methyltransferase-1 (DNMT-1) expression of eutopic endometrium via menstrual blood between endometriosis patients and non-endometriosis.
Patients and methods: We conducted a study of PR-B promoter methylation and mRNA DNMT-1 expression in menstrual blood obtained on the first and second day of the period from forty women with endometriosis and without.
Results: Methylation analysis and mRNA analysis revealed a significant difference in the level of DNA methylation of PR-B promoters in the endometriosis group compared to non-endometriosis (79.129%±4.719 vs 28.716% ± 4.732, p<0.05). DNMT-1 mRNA expression was higher, though statistically insignificant, in the endometriosis group than non-endometriosis (10.517±13.421 vs 9.532 ± 10.269, p > 0.05).
Conclusion: Correlation analysis showed a positive correlation between PR-B methylation and mRNA DNMT-1 expression in endometriosis. (r=0.526, p < 0.05).
Publisher
Research Square Platform LLC
Reference23 articles.
1. Modulation of estrogen receptor signaling by association with the activated dioxin receptor;Ohtake F;Nature,2003
2. Reduced expression of progesterone receptor-B in the endometrium of women with endometriosis and in cocultures of endometrial cells exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin;Igarashi TM;Fertil Steril Jul,2005
3. The link between exposure to dioxin and endometriosis: a critical reappraisal of primate data;Guo SW;Gynecol Obstet Invest,2004
4. The potential role of exposure to environmental toxicants in the pathophysiology of endometriosis;Rier SE;Ann N Y Acad Sci,2002
5. Endometriosis in Rhesus Monkeys (Macaca mulatta) Following Chronic Exposure to 2,3,7,8-Tetrachlorodibenzo-p-dioxin;Rier SE;Fundam Appl Toxicol,1993