Abstract
Abstract
Marchantia polymorphya is one of the earliest terrestrial plant. As a new type of model plant, there are many advantages in plant biological researching, such as short growth cycle, haploid gametophyte dominance, sexual and asexual propagation simultaneously, and so on. In this study, a non-tissue culture dependent genetic transformation was developed for M. polymorphya. This method consists of immersion of explants (thalli or mature gemmae collected in field conveniently, without pre-culture) in solution of Agrobacterium, inoculation on soil simply, screening by spaying of selection solution directly. Over-expression of MpCCAAT-NFYB5(Mp6g07410.1) and corresponding changes of phenotype were observed. Furthermore, non-chimera transformant could be obtained rapidly through four cycles of gemmae reproduction. This method will facilitate further molecular biological works in this model plant.
Publisher
Research Square Platform LLC
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