Bioprospecting of Actinomycetes from Diverse Ecosystems: A Potential Source of Pharmaceutically Active Metabolites

Abstract

Abstract Present work includes isolation of actinomycetes from marine, mangrove, and terrestrial environments followed by their screening for bioactivity. Study led to the isolation of 109 actinomycete isolates. Broth supernatants and ethyl acetate crude extracts were analyzed for bioactivity of which, 19 isolates showed a potent inhibition against E. coli, 17 isolates showed activity against S. aureus, 10 isolates exhibited activity against K. pneumoniae and B. subtilis each, and 9 isolates showed zone of inhibition against C. albicans. Potential actinomycetes were and identified by 16S rRNA gene analysis and their sequences were deposited in GenBank. Two isolates belonging to non-Streptomyces genera viz., Micromonospora and Nocardiopsis were obtained. The broad-spectrum antimicrobial activity against all the test organisms at very low concentration was shown by soil isolates D, D2, F30 (brown pigment), TS13 (Orange red), TS14 (green), TS4 (cherry red), and marine isolate M11 (yellow).