Abstract
Abstract
Background: Decoctions of root and stem of the medicinal plant Salacia reticulata is an indigenous remedy for diabetics and its complications in Sri Lanka. In diabetics, the formation of advanced glycation end products (AGEs) leads to many pathologies. Nevertheless, the anti-protein-glycation property of this plant is poorly documented. This study reports the anti-protein-glycation and radical scavenging potential of various plant parts of S. reticulata.
Methods: Hot water extracts (2g dried powder/50 ml) of root, stem, leaf, twigs, and fruits at various concentrations (15.6 to 500.0 µg/ml) were subjected to anti-glycation and glycation reversing assays in vitro. 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was used for free radical scavenging property.
Results: Various plant parts of S. reticulata showed anti-protein-glycation and free-radical scavenging activities. IC50 for the anti-glycation activity of root, stem, leaf, twigs, and fruits extracts were 11.92 ± 1.14, 35.18 ± 2.79, 113.3 ± 1.91, 149.59 ± 1.06, and 1120.37 ± 229.48 µg/ml respectively. IC50 of Rutin was 21.88 ± 2.82 µg/ml. EC50 of the root, stem, twigs, and leaf extracts for glycation reversing was 102.09 ± 6.23, 116.99 ± 5.82, 154.45 ± 5.79, and 278.78 ± 14.19 µg/ml respectively. The EC50 values for the radical scavenging activity of leaf, stem, and roots were 26.4±4.7, 9.0±1.2, and 9.1±1.3 respectively. Root had significantly (p<0.05) high activity for all the parameters tested.
Conclusion: Salacia reticulata possess anti-glycation, glycation-reversing and free radical scavenging activities. Other than root and stem, the leaves and twigs too may be a useful source for anti-diabetic bioactive molecules.
Publisher
Research Square Platform LLC
Reference30 articles.
1. Global epidemic of type 2 diabetes: implications for developing countries;Osei K;Ethn Dis,2003
2. American Diabetes Association. Diagnosis and Classification of Diabetes Mellitus. Diabetes care. 2010;33:S62-69. https://doi.org/10.2337/dc10-S062.
3. Diabetes and its drivers: the largest epidemic in human history?;Zimmet PZ;Clin Diabetes Endocrinol,2017
4. International Diabetes Federation (IDF). IDF Diabetes Atlas, 10th ed. IDF, 2021 update, Brussels, Belgium. https://diabetesatlas.org/.
5. Diagnosis and Classification of Diabetes Mellitus;American Diabetes Association;Diabetes Care,2014