Proteomic analysis of Aquilaria malaccensis seed in relation to seed viability

Abstract

Abstract Aquilaria malaccensis is one of the woody species and its economic value, high demand in the market, overexploitation, and difficulty in propagation due to the low shelf life of the seed cause the species to face the threat of extinction. The main objective of the study is to understand the role of protein in seed viability. In this experiment, the protein was extracted using tris- HCl/trichloroacetic acid-acetone (TCA-acetone) from the immature seed, premature seed, fresh mature seed, 7 days stored seed, 14 days stored seed, and 30 days stored seed. The extracted protein concentration was estimated using the Bradford method and separated using 1-D SDS PAGE. With the maturity of the seed concentrations of protein increase (immature seed 18.11 µg/ml, premature seed 37.43 µg/ml, and fresh mature seed 68.92 µg/ml ) and it reduces with storage (7 days 23.92 µg/ml, 14 days 20.14 µg/ml, and 30 days 16.08 µg/ml). Proteomic analysis using SDS PAGE, revealed that with the maturity of the seed, development protein was observed, immature seeds show no band, premature seeds showed four bands, and freshly matured seeds show 5 dark bands. The 7 days stored seeds show two bands 14 days stored seeds showed only one band, and no band was observed in 30 days stored seeds. Furthermore, the loss of seed proteins in seed storage might induce a loss of their functional properties in seed viability.