Deciphering the genetic battle: How Argentine Creole cattle challenge Rhipicephalus (Boophilus) microplus through their genome

Author:

Masagué María Florencia Ortega1ORCID,Bonamy Martín2,Cutullé Christian1,Giovambattista Guillermo2

Affiliation:

1. Instituto Nacional de Tecnologia Agropecuaria

2. Instituto de Genetica Veterinaria Ingeniero Fernando Noel Dulout

Abstract

Abstract

The common cattle tick Rhipicephalus (Boophilus) microplus causes severe limitations to livestock production. Bovine genetics could be a decisive component for the success or failure of control programs for ticks and diseases transmitted. The objective of this work was to detect chromosomal regions associated with host resistance to R. (B.) microplus through an associative mapping study using medium and high density microarrays in a population of Argentine Creole cattle. The phenotypic record of the number of ticks that completed their development on the host, after artificial infestations, was obtained in 231 animals during 2015 to 2020. Genomic DNA was extracted for genotyping from 192 animals using Affymetrix high (Axiom™ Bos 1) and medium density (ArBos1) microarrays. In an exploratory study, chromosomal regions containing putative quantitative trait loci (QTLs) were recognized on chromosomes 27, 11, 10, 9, 16, 13, 3, 19, 8 and 18, associated with the variation of R. (B.) microplus load. Gene ontology based on genes located on these regions revealed an enrichment of terms and pathways for the immune system, blood coagulation, tissue regeneration, endopeptidase activity and protein phosphorylation. The information obtained in this work constitutes a first report of QTLs for tick count in the Argentine Creole cattle, and establishes the basis for additional analyzes that would contribute to the identification of biomarkers related to resistance to the common bovine tick in Argentina.

Publisher

Research Square Platform LLC

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