Affiliation:
1. Okayama University of Science
Abstract
Abstract
Canine pulmonary adenocarcinoma (PAC) resembles human lung tumors in never-smokers, but it is rarer than human pulmonary adenocarcinoma. Therefore, research on canine PAC is challenging. In the present study, we successfully established various novel canine PAC cell lines from a single lesion in a dog, including two parent cell lines and fourteen cloned cell lines, and characterized their cellular properties in vitro. Several of these cell lines showed epithelial–mesenchymal transition (EMT)-like and/or cancer stem cell (CSCs)-like phenotypes. We additionally assessed the sensitivity of the cells to vinorelbine in vitro. Three clonal lines, two of which showed EMT- and CSC-like phenotypes, were resistant to vinorelbine. These novel canine PAC cell lines can be utilized in future research for understanding the pathogenesis and development of treatments for canine PAC.
Publisher
Research Square Platform LLC
Reference50 articles.
1. Wakelee, H.A., Chang, E.T,. Gomez, S.L., Keegan, T.H., Feskanich, D., Clarke, C.A., Holmberg, L., Yong, L.C., Kolonel, L.N., Gould, M.K. & West, D.W. Lung cancer incidence in never smokers. J. Clin. Oncol. 25, 472 – 428. DOI: https://doi.org/10.1200/jco.2006.07.2983 (2007)
2. Survey of animal neoplasms in Alameda and Contra Costa Counties, California. I. Methodology and description of cases;Dorn CR;J. Natl. Cancer Inst.,1968
3. Canine neoplasia in the UK: estimates of incidence rates from a population of insured dogs;Dobson JM;J. Small. Anim. Pract.,2002
4. Distribution of histopathologic types of primary pulmonary neoplasia in dogs and outcome of affected dogs: 340 cases (2010–2019);McPhetridge JB;J. Am. Vet. Med. Assoc.,2021
5. Griffey, S.M., Kraegel, S.A. & Madewell, B.R. Rapid detection of K-ras gene mutations in canine lung cancer using single-strand conformational polymorphism analysis. Carcinogenesis 19, 959–963, DOI: https://doi.org/10.1093/carcin/19.6.959 (1998).