Determination of Plant Regeneration Protocol of “Viking” Aronia Cultivar in the In Vitro Conditions

Abstract

Abstract In this study, in vitro micropropagation of 'Viking' aronia cultivar was investigated. For this purpose, different cytokinin and doses were tested in MS food medium for propagation medium. Except for cytokinins, low doses of IBA and GA were added and equal doses were used in all applications. For the rooting medium, different doses of IBA (0.125, 0.25, 0.5 and 1.0 mg l-1) and activated charcoal (0.5 and 1.0 g l-1) were tested in MS and ½MS mediums. A mixture of perlite and peat was preferred during acclimatization. No plant death was encountered in the study, and 100% viability was detected in all applications. The highest number of shoots was detected in media containing TDZ; however, most of the shoots that can be used as micro cuttings were obtained from media containing BA and mT. The highest number of shoots occurred at 1.0 mg l-1 doses of media containing BA and mT, and 16.83 and 21.76 shoots/explants were obtained, respectively. 100% rooting was achieved in all applications, including the control. The highest root number was detected in ½MS medium (10.17 roots/explant) containing 1.0 mg l-1 IBA. Higher root number was obtained in ½MS medium compared to MS medium; However, it was determined that the root length decreased with the increase in the number of roots. The acclimatization phase has resulted in high success, 98% of plants in ½MS medium and 92.4% of plants in MS medium survived.