Morphological and functional differences of monocyte derived dendritic cells in patients with acute coronary syndrome

Abstract

Abstract Objective To investigate the morphological and functional differences of monocyte derived dendritic cells in different types of ACS patients. Methods The peripheral blood mononuclear cells (PBMC) were isolated and differentiated into mononuclear dendritic cells (moDC) which morphology was observed by electron microscope and its related molecules (CD11c, CD14, CD80, CD86, HHLA -DR) were identified by flow cytometry (FCM) and the expression levels of IL6, IL10 and IL12p70 were detected by ELISA in acute coronary syndrome patients with unstable angina (UA), non-ST elevation myocardial infarction (NST), ST elevation myocardial infarction (ST) and healthy control group (CON). Results (1) FCM showed the moDC surface molecules CD11c and HLA-DR were highly expressed, while CD80 and CD86 were weakly expressed. (2) Electron microscopy showed the DC of UA, NST and ST groups had longer and more dense pseudopods than that of CON group. The expression levels of CD80 and HLA-DR in ST group and NST group were increased, while the expression levels of OVA were decreased compared with CON group and UA group,(p<0.05). (3) The levels of serum IL6 and IL12p70 in ST and NST groups were significantly higher than those in CON and UA groups (p<0.05), but there was no significant difference in the expression level of IL10 among the four groups. ConclusionsThe morphology and function of moDC in patients with different types of ACS are different. In patients with acute myocardial infarction (AMI), moDC has stronger antigen presenting ability, pro-inflammatory secretion function and weaker phagocytosis ability compared with non-myocardial infarction patients