Suggestion the role of antibodies/immunoglobulins, produced by non-lymphoid cells, tissues and organs, as participants in different regulatory mechanisms

Abstract

Abstract The main goal was related with a better understanding of the intra- and extra-cellular inter-molecular interactions in the different regulatory pathways, by which the separate cells, tissues and organs communicate to each other. The functions of tri-peptide Glutathione (GSH) as tumor suppressor, neuroprotector and endocrine regulatos, but also its role in the regulation of cellular growth, proliferation, differentiation and death, as well as of inflammatory and pro-inflammatory processes has been proved. In the current study, total extracts (controls) from rat brain and rat pancreas were prepared. Separate amounts were passed through GSH-agarose columns to select molecules, possessing affinity to GSH. The average titers of gangliosides and of anti-ganglioside antibodies in the so prepared biological samples were determined by enzyme-linked immunosorbent assay (ELISA). Possibility about production of immunoglobulins/antibodies by non-lymphoid cells, tissues and organs was suggested, probably as one of the steps in the regulatory pathways. Because the so produced antibodies are outside the germinative centers of the specialized lymphoid tissues and organs, control of their function by small ions and molecules as gangliosides is very important, by direct intra- and extra-cellular inter-molecular interactions or by indirect influence on various regulatory mechanisms.