Exploring Genetic Alterations in Hodgkin Lymphoma using Comparative Genomic Hybridization on DNA Microarrays

Abstract

Abstract The genetic alterations can contribute to the development and progression of the disease. In the case of c HL, a type of cancer that affects the immune system, understanding the genetic changes that occur may inform the development of more targeted and effective treatments. In this study, we used comparative genomic hybridization CGH on DNA microarrays to perform a whole-genome analysis of 30 individuals, 15 patients with HL and 15 healthy controls. Aberrations were divided into three groups based on size: large aberrations, regional aberrations, and microstructural aberrations Our results showed that there were more duplications of genetic material (gains) than deletions (losses), and that specific genetic additions and deletions were present in multiple tumors. Aberrations were divided into three groups based on size: large, regional, and microstructural. Large aberrations included trisomy of whole chromosomes and gains or losses of chromosome arms. Regional aberrations were greater than 5 Mb in size and were found on multiple chromosomes. Microstructural aberrations were smaller than 5 Mb and had high amplitudes. Amplification of specific loci, such as 5q31.1 and 8q24.3, were found in multiple tumors, and loss of a single region, such as 8q22.1, was found in multiple tumors as well. it appears that genetic alterations of chromosome 8, specifically the 8q21-8q22 and 8q24.2 regions, are common in individuals with Hodgkin lymphoma. In particular, the MYC and MALT1 genes, as well as the RAD54B protein, may play a role in the development of Hodgkin lymphoma. and could potentially be targeted for therapeutic purposes. Overall, the results of this study provide insight into the genetic alterations present in Hodgkin lymphoma and may inform future research and treatment approaches. Further research is needed to fully understand the functional consequences of these genetic changes and to explore their potential as therapeutic targets. Exploring Genetic Alterations in Hodgkin Lymphoma using Comparative Genomic Hybridization on DNA Microarrays