The Complete Genome Sequence of Clade B, Wheat Streak Mosaic Virus Isolate from Turkey

Abstract

Abstract Wheat streak mosaic virus is one of the most widespread viruses in cereal crops, causing severe losses, dramatically affecting worldwide wheat production. Currently, four distinct clades of WSMV have been grouped and named: A (Mexico), B (Europe, Asia, Russia), C (Iran), and D (United States, Argentina, Brazil, Australia, Canada, Turkey). Each of these groups is based on genome-wide variability, emphasizing the CP. Previously reported Turkish wheat isolates of WSMV clustered within both clades D and B. However, the placement of the Turkish WMSV into clade B is only based on a partial genome sequence. Here, we used high throughput sequencing to assemble the complete genome sequence of WSMV type B isolate collected from wheat found in the European part of Turkey. Excluding the poly(A) tail, the genome of isolate S34Edirne (Genbank no. MZ405098) consists of 9,360 nucleotides and contains a single large open reading frame encoding a polyprotein of 3,033 amino acids. The characteristic lack of a GAG (Gly2761) codon within the CP of the polyprotein is typical for the clade B, WSMV-ΔE isolates, which are widely found throughout the European continent. However, two American isolates were recently placed in this group. Sequence comparisons revealed that WSMV Turkish wheat isolate is the most closely related to Czech isolate, with highly similar nucleotide and amino acid identities at 98.83-99.13%, respectively. The result of this study indicates that the WSMV full-length genome of S34Edirne isolate should be placed into clade B of the European WSMV-ΔE isolates.