Low-temperature plasma irradiation of Ringer`s lactate generates heterogeneous molecules for cancer treatment-Reference-Cited by-同舟云学术

Low-temperature plasma irradiation of Ringer`s lactate generates heterogeneous molecules for cancer treatment

Published:2022-09-02 Issue: Volume: Page:
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Author:

Miron Camelia¹,Kashiwagura Satoshi¹,Britun Nikolay¹,Ito Daiki¹,Iwata Naoyuki¹,Liu Yang¹,Kajiyama Hiroaki²,Toyokuni Shinya²,Mizuno Masaaki³,Hashizume Hiroshi¹,Kondo Hiroki¹,Ishikawa Kenji¹,Tanaka Hiromasa¹,Hori Masaru¹

Affiliation:

1. Center for low-temperature plasma sciences, Nagoya University

2. Nagoya University Graduate School of Medicine

3. Nagoya University

Abstract

Abstract Low-temperature plasma (LTP) is a promising tool for cancer treatment because irradiated various solutions show selective antitumoral effects on cancer cells. In this study, Ringer`s lactate solution was irradiated by pulsed electrical discharges ignited in argon, nitrogen, and oxygen gas mixtures. The chemical compounds formed by LTP and their effects on non-tumorigenic breast epithelial cells (MCF-10A) and breast cancer cells (MCF-7) were investigated. Among these compounds, glyceric acid increased the cell viability by more than two-fold compared to the control samples for MCF-10A and MCF-7 cells, whereas the tricarballylic acid had a pronounced cytotoxic effect on the cells when incubated with solutions of 0.6 mM to 50 mM concentrations. The results show that the LTP-generated chemical compounds have both, stimulatory and inhibitory effects on cell viability, possibly by influencing the morphology of the cells and physiological functions, depending on the concentration of the generated compounds in the irradiated liquids.

Publisher

Research Square Platform LLC

Reference36 articles.

1. 1. Tanaka, H., Mizuno, M., Ishikawa, K., Nakamura, K., Kajiyama, H., Kano, H.; Kikkawa, F. & Hori, M. Plasma-Activated Medium Selectively Kills Glioblastoma Brain Tumor Cells by Down-Regulating a Survival Signaling Molecule, AKT Kinase. Plasma Med. 1, 265–277 (2011).

2. 2. Iwasaki, M., Inui H., Matsudaira, Y., Kano, H., Yoshida, N., Ito, M. & Hori, M. Nonequilibrium atmospheric pressure plasma with ultrahigh electron density and high performance for glass surface clea-ning. Appl. Phys. Lett., 081503 (2008).

3. 3. Schlegel, J., Köritzer, J. & Boxhammer, V. Plasma in cancer Treatement. Clin. Plasma Med. 1, 2–7 (2013).

4. 4. Laroussi, M. Cold Plasma in Medicine and Healthcare: The New Frontier in Low Temperature Plasma Applications. Front. Phys. 8, 74 (2020).

5. 5. Lin, A., Truong, B., Patel, S., Kaushik, N., Choi, E. H., Fridman, G., Fridman, A. & Miller, V. Nanosecond-Pulsed DBD Plasma-Generated Reactive Oxygen Species Trigger Immunogenic Cell Death in A549 Lung Carcinoma Cells through Intracellular Oxidative Stress. Int. J. Mol. Sci. 18, 966 (2017).