The influence of different arterial carbon dioxide levels on the cerebrovascular autoregulation curve in a porcine cranial window model.

Abstract

Introduction: Cerebrovascular autoregulation (CA) is the ability to maintain adequate cerebral blood flow (CBF) over a wide range of arterial blood pressure (ABP). Carbon dioxide (PaCO₂) is a potent vasodilator, but its precise influence on CA remains incompletely understood. Methods: A porcine cranial window model, in which CBF can be measured directly in the pial arterioles while ABP is mechanically manipulated, is used to investigate the effect of PaCO₂ on CA capacity. Hypercapnia and hypocapnia were induced in 12 male piglets each by adjusting the respiratory rate. Once stable PaCO₂ levels of resp 60 mmHg and 25 mmHg respectively were achieved, ABP was gradually increased in half of the animals or decreased in the other half beyond limits of CA by inflating a balloon in the aorta or inferior caval vein. Results: Before ABP manipulation, hypercapnia already induced a significant vasodilation (+33.9%) and increase in CBF (+20.5%) whereas hypocapnia did not alter diameter or CBF. Both hyper- and hypocapnia significantly reduced the ability to adjust arteriolar diameters in response to changes in ABP. Conclusion: During hypo- as well as hypercapnia, narrowing of the CA range with a shorter plateau between upper and lower limit of autoregulation was observed, compared to normocapnia.