Multidimensional and comprehensive single-cell analysis of the effect of PBK on immune infiltration in lung adenocarcinoma

Abstract

Abstract Phosphorylation is essential for tumor-induced angiogenesis, but how it affects angiogenesis remains unclear. We introduced phosphorylation-related genes from the lung adenocarcinoma dataset, screened out the most characteristic genes PBK through various machine learning methods, and repeatedly verified their survival data, mutation frequency, and clinical information. Subsequently, we conducted a comprehensive analysis of whole cancer based on lung adenocarcinoma, analyzed 33 cases of pan-cancer by GSEA enrichment, screened out a variety of angiogenesis-related pathways, and analyzed the correlation between related risk difference genes and pan-cancer PBK. Finally, we cultured HUVEC cells to simulate the tumor microenvironment and verify cell phenotypic experiments such as cell proliferation, migration, and cell cycle. Finally, it was proved that PBK can affect tumor angiogenesis and the differentiation of Treg cells, and the study flow chart is shown in Fig. 1 for reference only.