Heimdall, an alternative protein issued from a ncRNA related to kappa light chain variable region of immunoglobulins from astrocytes : a new player in neural proteome

Author:

Capuz Alice1,Osien Sylvain1,Tristan Cardon1,karnoub Melodie1,Aboulouard Soulaimane2ORCID,Romero Antonella Raffo3,Duhamel Marie4ORCID,Cizkova Dasa5ORCID,Trerotola Marco6,Devos David7,Kobaissy Firas8ORCID,Abeele Fabien Vanden9,Bonnefond Amelie10,Fournier Isabelle11,Rodet Franck2ORCID,Salzet Michel1ORCID

Affiliation:

1. Unversité de Lille, Inserm, CHU Lille

2. University of Lille

3. Université de Lille 1

4. Univ.Lille, Inserm, CHU Lille, U1192, Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), F-59000 Lille, France

5. Institute of Neurobiology, Slovak Academy of Sciences

6. University of Chieti

7. Université de Lille, INSERM

8. Morehouse School of Medicine

9. Inserm U-1003, Equipe labellisée par la Ligue Nationale contre le cancer, Laboratory of Excellence, Ion Channels Science and Therapeutics, Université Lille 1, Cité Scientifique, 5

10. Université de Lille

11. Univ. Lille, Inserm, CHU Lille, U1192 - Protéomique Réponse Inflammatoire Spectrométrie de Masse - PRISM

Abstract

Abstract The dogma “One gene, one protein” is clearly obsolete since cells use alternative splicing and generate multiple transcripts which are translated into protein isoforms, but also use alternative translation initiation sites and termination sites on a given transcripts. Alternative open reading frames for individual transcripts give proteins (the alternative Proteins: AltProts) originate from the 5'- and 3'- UTR mRNA regions, frameshifts of mRNA ORFs or from non-coding RNAs. To gain insight into the role of these newly identified alternative proteins in the regulation of cellular functions, it is crucial to assess their dynamic modulation within a framework of altered physiological modifications such as experimental spinal cord injury (SCI). Here, we carried out a longitudinal proteomic study on rat SCI from 12h to 10 days. Based on AltProt predictions, it was possible to identify a plethora of newly predicted protein hits. Among these proteins, some presented a special interest due to high homology with variable chain regions of immunoglobulins. We focus our interest on the one related to Kappa variable light chains which is similarly highly produced by B-cells in the Bence jones disease, but here expressed in astrocytes. This protein, name Heimdall is an Intrinsically disordered protein which is secreted under inflammatory conditions. Immunoprecipitation experiments showed that the Heimdall interactome contained proteins related to astrocyte fate keepers such as “NOTCH1, EPHA3, IPO13”. However, when Heimdall protein was neutralized utilizing a specific antibody or its gene knocked out by CRISPR-Cas9, sprouting elongations were observed in the corresponding astrocytes. Interestingly, depolarization assays and intracellular calcium measurements in Heimdall KO, established a depolarization effect on astrocyte membranes KO cells impacting the astrocyte phenotype sustained by the decrease of NOTCH2. Taken together, Heimdall is a novel neural key player involved in astrocytes gatekeeper phenotype.

Publisher

Research Square Platform LLC

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