Identification of Ferroptosis-related genes in sepsis-induced acute respiratory distress syndrome

Abstract

AbstractObjective:The objective of this investigation was to examine the association between genes related to ferroptosis (FAGs) and acute respiratory distress syndrome (ARDS) induced by sepsis in individuals.Methods:Microarray profiles (GSE332707) from the GEO database were utilized to screen for differential genes. FAGs were derived from three gene pools (KEGG database, NCBI, FerrDb database). The co-expression genes were obtained through the intersection of differential genes (DEGs) in GSE332707 and FAGs. Subsequently, hub genes were discerned by means of GO, KEGG, and PPI network analysis. The validation of these hub genes was carried out experimentally using the RT-qPCR technique and a separate dataset (GSE66890). Ultimately, target gene prediction was conducted through the utilization of GeneCard and StarBase 3.0.Results:A total of 565 DEGs were identified between sepsis-induced ARDS and control samples, with 30 co-differential genes being detected. Then, the protein interaction network of 30 co-differential genes unearthed 5 hub genes (CTSB, LCN2, ZFP36, KLF2, and IRF1). Validation of the 5 hub genes was performed using RT-qPCR and GSE66890, which confirmed LCN2 as a potential prognostic candidate gene. The hsa-miR-374b-3p emerged as the most strongly supported candidate miRNA of LCN2.Conclusion:Based on our findings, we conclude that LCN2, a potential biomarker associated with FAGs, may play a role in the pathogenesis of sepsis-induced ARDS.