Evaluation of Circulating Levels of miR-135a and miR-193 in patients with sepsis

Abstract

Abstract Background Sepsis is a life-threatening condition that the early diagnosis and prognostic awareness provide direction for selecting the appropriate treatment strategies. A wide variety of biomarker-based studies in clinical medicine provide new insights into the personalized medicine in sepsis patients. MiRNAs are endogenous non-coding RNA molecules which have been acting as the great potential diagnostic, prognostic and therapeutic biomarkers in diseases. Methods and Results In present study, the expression levels of two selected miRNAs, including miR-135a and miR-193, were evaluated for their prognostic potential in patients with sepsis. The circulating levels of miRNAs were quantified by quantitative PCR (qPCR) in patients with sepsis (n = 100) and age- and sex-matched healthy controls (n = 100). Statistical findings confirmed the valuable prognostic potential of miR-135a in patients with sepsis, while no significant difference was found between the miR-193 expression level in the patients with sepsis and controls. Conclusions Circulating level of miRNA-135a can serve as the prognostic biomarker for patients with sepsis. These findings highlight the importance of miRNAs as signatures in the personalized managements of sepsis.