Effect of food intake on 143 biomarkers measured by Proseek Multiplex Inflammation I and Multiplex Organ Damage panels

Abstract

Abstract Objective This study investigates the effect of food intake on 143 different biomarkers. Methods Twenty-two healthy subjects (11 male and 11 female aged 25.9 ± 4.2 years) were investigated. A total of 143 biomarkers were measured before a standardized meal as well as 30 and 120 minutes afterwards with the Proseek Multiplex Inflammation I, and Multiplex Organ Damage panels. Results The levels for 23 biomarkers were significantly (p < 0.001) changed due to food intake. A total of 14 biomarkers decreased 30 minutes and 120 minutes after food intake. Four biomarkers were increased only at 120 minutes after food intake. The changes for the biomarkers were between 2% and 105%. Conclusion This study shows that food intake has some effect on 143 different biomarkers. The timing of blood sampling in relation to food intake appears not to be a major concern. There are some exceptions. Standardization concerning food intake should be attempted when investigating Interleukin-6, Anterior gradient protein 2 homolog, BH3-interacting domain death agonist, Tyrosine-protein kinase Fes/Fps, Syntaxin-8, Probetacellulin, Peptidyl-prolyl cis-trans isomerase FKBP1B, Ribonucleoside-diphosphate reductase subunit M2 B, and Enteropeptidase which all changed more than 30%.