Abstract
Background: Pediatric stroke is a significant cause of childhood mortality and morbidity. The clinical research in this field bears certain limitations that do not exist in the pre-clinical setting. In pre-clinical research, experimental models of ischemic stroke show differences in lesion evolution and blood-brain barrier (BBB) permeability between adult and neonatal rats. However, little is known about these factors in the juvenile stage.
Aims: To characterize the evolution of the lesion, penumbra and degree of BBB permeability in a photothrombotic ring model of juvenile stroke.
Methods: The design is a mixed longitudinal and cross-sectional study. In 14 Sprague Dawley juvenile rats (weight 130-189 g), lesion, penumbra volume and blood-brain barrier (BBB) leakage were measured longitudinally on days 0, 2, and 7 following photothrombotic stroke. Magnetic resonance imaging (MRI) techniques were conducted to measure lesion and penumbra volumes (T2-weighted imaging [T2] and water restriction (diffusion-weighted imaging [DWI]) and BBB leaking (with dynamic contrast-enhanced imaging [DCE]). Histology was performed to confirm stroke (n=9) with Triphenyltetrazolium chloride staining (TTC); (n=3) for Haemotoxylin and Eosin (H&E) staining; and (n=9) Evans Blue (EB) staining to assess BBB permeability.
Results: We found the penumbra volume to be larger and better delineated on MRI and histology in the acute compared to the subacute and chronic stages, and the lesion to be smaller in volume, increasing over time following same time trajectory. The BBB was most compromised at the hyperacute stage (day 0) and decreasingly, yet persistently, disrupted to day 7.
Conclusions: Our in vivo and ex vivo findings provide insight into the evolution of stroke and could serve as a study model to test blood-brain barrier stabilization agents in the pediatric setting.