Protective Effects of Curcumin/Magnesium Oxide Nanoparticles Against Ketamine- induced Neurotoxicity

Author:

Salehirad Mahsa1,Hayes A. Wallace2,Motaghinejad Majid3,Gholami Mina3

Affiliation:

1. Tehran Medical Sciences, Islamic Azad University

2. Michigan State University

3. Shahid Beheshti University of Medical Sciences

Abstract

Abstract A curcumin-conjugated MgO nanostructure (Cur/MgO NPs) was synthesized, and its composition was verified. XRD and a particle size analyzer were used to determine the average crystalline and particle sizes. Morphological studies were conducted using FE-SEM. UV-Vis was also employed to examine absorption patterns, and FT-IR spectroscopy analyzed the functional groups involved in the reaction. The following protocol evaluated the effectiveness of Cur/MgO NPs in ketamine-treated male BALB/c mice. Group 1 received 0.2 mL of normal saline. Group 2 animals received Ket (25 mg/kg). Group 3 animals received 40 mg/kg Cur and 25 mg/kg Ket. Groups 4–6 received Ket (25 mg/kg) and Cur/MgO N.P.s (10, 20, or 40 mg/kg). Group 7 received 5 mg/kg MgO and Ket (25 mg/kg). Mice were injected ip daily for two weeks. The hippocampal tissue was analyzed for oxidative stress, inflammation, apoptotic markers, and mitochondrial quadruple complex enzymes. The Cur/MgO N.P.s were neuroprotective against the inflammation, apoptosis, and oxidative stress induced by Ket.

Publisher

Research Square Platform LLC

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