Genetic diversity analysis of Rumex patientia L. in different habitats

Abstract

Abstract Objective The present study was undertaken to investigate the genetic diversity of Rumex patientia L. in distinct habitat types. Methods Eighty samples from 4 natural populations in Jilin Province of China were analyzed by inter-simple sequence repeats (ISSR). Results ISSR techniques produced 135 bands and 71 polymorphic bands, of which the polymorphic bands (PPB) accounted for 52.6% among natural populations; the gene diversity (H) was 0.1751, and Shannon's diversity index (I) was 0.2634. In the population, the percentage of polymorphic loci (P) was 25.93% and 37.04%, the genetic diversity index (H) was 0.0714–0.1124 and Shannon’s diversity index (I) was 0.1121–0.1676. Population E had the highest genetic diversity, however, population S had the lowest. Based on the species genetic differentiation analysis, the total Nei diversity index (Ht) of Rumex patientia L. from Jilin City was 0.1110 and the within-population genetic diversity (Hs) was 0.1751. The coefficient of genetic differentiation between population areas (GST) was 0.3662. The Gene flow between populations was 0.8654. Conclusion The results showed that the inter-population variation accounted for 36.62% of the total variation, and the intra-population variation accounted for 63.38%. Cluster analysis based on Nei’s genetic distance showed that the greatest genetic distance between E and S (0.1218) and the closest genetic distance between W and N (0.0794).