Abstract
The study aimed to compare lipid proton concentrations across the 3 fatty liver models, highlighting induction methods and differences in hepatic protonation identified through Magnetic Resonance Spectroscopy (MRS) analysis to gain insights into fatty liver pathogenesis and potential therapeutic approaches. This research sought to induce various conditions of fatty liver and examine disparities in hepatic protonation patterns through meticulous MRS analysis. The models for fatty liver in mice were established using distinct methods including ethanol feeding, MCD diet, and CCl4 injection. MRS was utilized for lipid proton analysis, with data processed using LCModel software. Statistical analyses encompassed profile comparisons via OPLS-DA, consistency evaluations using Kendal correlation, assessments of concentration differences via Mann-Whitney U test, and an exploration of lipid accumulation influences through Spearman's correlation analysis. All three fatty liver models displayed hepatic fat infiltration rates exceeding 40%, with the MCD model showing the highest rate at 52.46%. Analyzing the lipid protons, a higher concentration of methylene protons was observed in the NIAAA model compared to the other groups. Additionally, there were notable distinctions in the composition values of specific lipid protons across the models, highlighting varying trends in fatty acid deposition. The correlation and composition analysis provided insights into the different patterns of lipid accumulation in each model, with significant correlations identified among various lipid protons. Furthermore, differences in the composition values of specific lipid protons indicated distinct characteristics of fatty acid profiles in the different fatty liver models. There is no significant differences were found between the NIAAA and CCl4 models, but the MCD model exhibited higher deposition of polyunsaturated bonds, specifically diallylic protons, compared to other groups.