Pathological Changes and Immunohistochemical Detection of Encephalitozoon cuniculi of Fox Origin

Abstract

Abstract Encephalitozoon cuniculi is an obligate intracellular parasitic eukaryotic pathogen.The blue fox (Alopex lagopus) is one of the most susceptible species of microsporidian in rabbit encephalitis. In this study, the kidney, brain, heart, liver, spleen and intestine tissues of diseased and healthy blue foxes were collected, and paraffin sections were prepared by H&E staining. The pathological changes of each tissue were observed under a microscope, photographed and pathological analysis was performed, and the results were found by comparison The fox’s kidney and brain are susceptible tissues of microsporidian rabbit encephalitis. The typical pathological changes are chronic interstitial nephritis and diffuse non-suppurative meningoencephalitis. Heart and liver tissues occasionally have inflammatory lesions, while the spleen and There is no obvious lesion in the intestinal tissue. The kidney and brain tissues, which are susceptible to the disease, were selected for immunohistochemical tests. The fox serum was purified by affinity chromatography to prepare fox IgG, fox IgG was used as the primary antibody, rabbit anti-fox IgG was used as the secondary antibody, DAB was used as the developer, and hematoxylin staining was used to establish an immunohistochemical detection method to locate rabbit encephalitis microbes. Sporozoite antigen. The spores of Microsporidium encephalitis were observed in the kidney and brain tissues of sick foxes by immunohistochemistry. The spores of rabbit encephalitis were brown and granular. In the kidney tissue, the spores were mostly located in the renal capsule and tubule, while in the brain tissue only A few spores were found. Through the preparation of paraffin sections, the histopathological changes of the blue fox infected with Microsporidium encephalitis in rabbits were analyzed specifically to provide guidance for the diagnosis of the disease. The immunohistochemical method established in this study shows that it is more specific than histopathological diagnosis, can accurately locate the location of microsporidium, and is suitable for the detection of fox-derived rabbit encephalitis microsporidia.