The Prognostic and Immunotherapeutic Significanceof NCAPD2 in Pan-Cancer, and Its Relationship With the Proliferation and Metastasis of Hepatocellular Carcinoma

Abstract

Abstract Background Present studies indicated that NCAPD2 has emerged as an essential participant of condensin I involved in the mitotic chromosome assembly and dissociation. Simultaneously, NCAPD2 has an enormous potent to interpret the tumor progression in a broader perspective. Due to the superficial comprehensive analysis of NCAPD2 in pan-cancer, lacking of detailed studies was to explore the underlying pathogenesis and the potential regulatory mechanism. Herein, this study systematically provided the prognostic landscape visualization of NCAPD2 in pan-cancer, and further investigate the function and the correlation with immune infiltration characteristics in hepatocellular carcinoma (HCC). Method In this study, using the online databases TCGA, TIMER2.0, OPENTARGET, GeneMANIA, HPA, UCSC, TCIA. This analysis suggested that the relevance between NCAPD2 and oncogenesis, progression, prognosis, methylation, immune infiltration and function. Subsequently, RT-qPCR, western blotting assays were performed to detect the NCAPD2 expression. Additionally, the proliferation of NCAPD2 silenced cells was evaluated using BrdU assay. Transwell assays further confirmed the effect of knock down NCAPD2 on migration and invasion capacity. Flow cytometry assay was finally performed to assess cell cycle and apoptosis in indicated cells. Result NCAPD2 was aberrant active in multiple cancers. NCAPD2 upregulation was related with poor prognosis in pan-cancer. Meanwhile, NCAPD2 gene mutation, the methylation level of NCAPD2 promoter and MSI increase occurred in a series of cancers. In addition, the results exhibited that a significant association between abnormal expression of NCAPD2 and immune infiltration cells and immune therapy response in HCC. Furthermore, this study clarified the utility of NCAPD2 that knock down its expression could result in suppressing proliferation, invasion and migration capacity, and induce the accumulation of G0/G1 cells and the apoptosis occurrence in HCC cells. Conclusion This study revealed that NCAPD2 could participate in the carcinogenic progression of various cancers. It used as a potential diagnostic and prognostic marker in pan-cancer, especially HCC patients, and contributed to the NCAPD2-based estimation of immune-therapy response for HCC, which might provide a new strategies into immune therapy and precision medicine.