Evaluation of KLF4 Gene and Long Non-coding RNA MIR503HG Expression in Breast Cancer Patients in Iran

Abstract

Abstract Introduction: Breast cancer is the most common cancer among women and its mortality rate is high. Therefore, it is necessary to study the genes involved in cancer in order to better understand the molecular mechanism of this disease. Since gene expression and its regulation process in tumor tissues are different from normal tissues, therefore the purpose of this research is to investigate the expression level of lncRNA MIR503HG and KLF4 gene in breast cancer samples and also the possibility of using them as biomarkers were investigated. Also bioinformatically, the interaction of MIR503HG and KLF4 was investigated. Material and methods: In order to investigate about the changes in expression of LncRNA MIR503HG and KLF4 gene in 23 samples of breast tumor tissue and 23 samples of healthy adjacent tissue, first total RNA was extracted from the samples and then cDNA synthesis was performed. Finally, the Real-time PCR technique was used. The LncRRIsearch web server was used to investigate the interaction between MIR503HG and KLF4. Results: It was shown that the expression of KLF4 gene in breast tumor tissues is significantly reduced compared to healthy adjacent tissue (p-value = 0.0068). Expression of MIR503HG in breast tumor tissues is significantly increased compared to adjacent healthy tissue (Pvalue = 0.0075). Also, in examining the biomarker potential of KLF4 and MIR503HG genes, both were included in the category of acceptable biomarkers (AUC = 0.7). It was also bioinformatically predicted that the KLF4 gene and LncRNA MIR503HG interact.