Organ agar serves as physiologically relevant alternative for in vivo colonization

Author:

Pearson Melanie1ORCID,Shea Allyson2ORCID,Smith Sara1,Mobley Harry1ORCID,Forsyth Valerie1,Pahil Sapna3

Affiliation:

1. University of Michigan

2. University of South Alabama

3. Postgraduate Institute of Medical Education and Research

Abstract

Abstract Animal models for host-microbial interactions have proven valuable, yielding physiologically relevant data that may be otherwise difficult to obtain. Unfortunately, such models are lacking or nonexistent for many microbes. Here, we introduce organ agar, a straightforward method to enable the screening of large mutant libraries while avoiding physiological bottlenecks. We demonstrate that growth defects on organ agar were translatable to colonization deficiencies in a murine model. Specifically, we present a urinary tract infection agar model to interrogate an ordered library of Proteus mirabilis transposon mutants, with accurate prediction of bacterial genes critical for host colonization. Thus, we demonstrate the ability of ex vivo organ agar to reproduce in vivo deficiencies. This work provides a readily adoptable technique that is economical and uses substantially fewer animals. We anticipate this method will be useful for a wide variety of microorganisms, both pathogenic and commensal, in a diverse range of model host species.

Publisher

Research Square Platform LLC

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