A new hybrid β-cyclodextrin/PAMAM G.0 as an electrochemical sensor for isoniazid detection

Abstract

Abstract βcyclodextrin (β-Cd) was functionalized with polyamide dendrimer PAMAM G.0 and the formed material was used to adsorb copper and in a subsequent reaction with potassium hexacyanoferrate (II) forming a mixed valence complex on the on β-cyclodextrin/PamamG.0 surface (β-Cd-PAMAM-Cu/Fe). This material and its precursors were characterized by Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD) and by X-ray Photoelectron Spectroscopy (XPS). The β-Cd-PAMAM-Cu/Fe material was successfully tested in the electrocatalytic oxidation of isoniazid (ISO) using Cyclic Voltammetry (CV) and Square Wave (SW). The electrochemical performance of the modified sensor exhibited a limit of detection (LOD) of 3.69×10− 7 mol L− 1 with a linear range from 1.0×10− 6 mol L− 1 to 1.0×10− 5 mol L− 1 of isoniazid using CV. A second region was detected with a limit of detection of 1.98×10− 5 mol L− 1 with a linear range from 6.0×10− 5 mol L− 1 to 9.0×10− 4 mol L− 1 of isoniazid using CV. In the same way, two linear regions were obtained using SWV, whose LOD was 6.49×10− 9 mol L− 1 in the concentration range of 5.0×10− 7 to 1.0×10− 6 mol L− 1 and the second region presented a LOD of 1.96×10− 7 mol L− 1 in the concentration range of 2.0×10− 6 to 1.0×10− 5 mol L− 1. Recoveries ranging from 97.20 to 100.57% were calculated, demonstrating satisfactory accuracy of the proposed method.