Chromatographic evaluation of Safranal and Ellagic Acid in Ayurvedic Tea Formulation and its major Ingredients

Abstract

Abstract India is blessed with an extensive variety of plants, trees, shrubs, and herbs that are utilized in the traditional Indian medical system and have been reported to have excellent therapeutic effects in treating ailments. Historically, medicinal plants have been a primary source of compounds used in medicine to treat illnesses. In the indigenous medical system, Terminalia arjuna and Crocus sativusare the most beneficial and well-recognized medicinal plants for treating a wide range of serious illnesses. The goal of this study is thedevelopment of comparative chromatographic profiles ofAyurvedic tea formulation and its major ingredients for identification, and quantitative estimation of concerned PRS/Markers using HPTLC and HPLC. In this study, we have done HPTLC Fingerprint profiling of Ayurvedic tea formulation, Terminalia arjuna, Crocus sativus, Ellagic acid and Safranal.Safranal and Ellagic acid reference standards were visible in test solution of Crocus sativus extract, Terminalia arjuna and Ayurvedic tea formulation respectively and found comparable under UV light and after derivatization with derivatizing reagent. The quantification of Terminalia arjuna and Crocus sativus in formulation was done through HPLC. The quantitative evaluation of Safranal present in Ayurvedic tea formulation and Crocus sativus was 0.0330% and 0.1041% respectively. The quantitative evaluation of Ellagic acid present in Terminalia arjuna,stem bark and Ayurvedic tea formulation was 0.2814% and 0.1293% respectively.