Downregulation hsa_circ_0006620 inhibit the malignant progression of prostate cancer by regulation miR-502-3p/HK2 axis mediated aerobic glycolysis

Abstract

Abstract Background: Circular RNAs (circRNA) are a class of covalently closed single-stranded RNAs that have been implicated in cancer progression by regulation metabolism. But, the circRNA roles in prostate cancer remains unknown. Methods: This research aimed to use FISH and RT-qPCR to investigate hsa_circ_0006620 expression in prostate cancer cells and tissues after high-throughput sequencing. Our team made the luciferase reporter assay to validate hsa_circ_0006620 downstream target. Transwell migration assay, 5-ethynyl-20-deoxyuridine, and cell counting kit-8 were applied to investigate both proliferation and migration. In vivo tumorigenesis and metastasis assays were performed to investigate the hsa_circ_0006620 role in prostate cancer. Results: The outputs elucidated that hsa_circ_0006620 expression incremented in prostate cancer cells and tissues. Hsa_circ_0006620 downregulation inhibited prostate cancer cell proliferation as well as migration in in vivo and in vitro experiments. The luciferase results validated that miR-502-3p and HK2 were hsa_circ_0006620 downstream targets. HK2 overexpression or miR-502-3p inhibition reversed prostate cancer cell migration after hsa_circ_0006620 silencing. The study also found that overexpression of HK2 or inhibition of prostate cancerreversed aerobic glycolysis after hsa_circ_0006620silencing. Conclusion: Results demonstrate that hsa_circ_0006620 downregulation inhibits prostate cancer malignant progression by regulation miR-502-3p/HK2 axis mediated aerobic glycolysis.