Micropropagation of Ilex cassine ‘angustifolia’

Abstract

Abstract For mass propagation of Ilex cassine ‘angustifolia’, tissue culture was used to shorten its propagation cycle. We sterilized its nodal segments as explants and then cultured them on MS basic medium supplemented with different concentrations of 6-BA and NAA, and placed them in the culture room for the induction and proliferation of adventitious shoots. The highest induction rate of MS medium with 6-BA 1.0 mg/L and NAA 1.0 mg/L was used for the induction of adventitious buds in Ilex cassine ‘angustifolia’ nodal segments, reaching 80%. Buds sprouted on the 10th day, while the average value of bud length reached 1.62 cm at 30 days. The adventitious buds grew fast and robust. The best proliferation coefficient was 5.7 when explants were cultured on MS basic medium containing 6-BA mg/Land NAA 0.5 mg/L. For adventitious root induction, the adventitious shoots were transferred to MS basic medium with different concentrations of IBA and NAA. The results showed that the highest rooting rate of 98.7% was achieved when explants were cultured on MS basic medium supplemented with NAA 0.5 mg/L and IBA 1.0 mg/L, and the adventitious roots induced were thick and robust. Rooted sterile seedlings had been planted in the substrate and transferred to a greenhouse with sprinkler irrigation for further cultivation. The survival rate was about 90%.