A Dual Functional Ti-Ga Alloy: Inhibiting Biofilm Formation and Osteoclastogenesis Differentiation via Disturbing Iron Metabolism

Author:

Li Fupeng1,Huang Kai2,Yuan Kai2,Yang Yiqi2,Liu Yihao2,Zhou Xianhao2,Kong Keyu2,Yang Tao3,He Jian4,Ao Haiyong5,Liu Qian6,Tang Tingting2,yang shengbing2

Affiliation:

1. Shanghai Red Cross Ninth People's Hospital: Shanghai Jiao Tong University School of Medicine Affiliated Ninth People's Hospital

2. Shanghai Jiao Tong University School of Medicine Affiliated Ninth People's Hospital

3. City University of Hong Kong

4. Duke Medicine

5. East China JiaoTong University

6. Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital

Abstract

Abstract Background Although biomedical implants have been widely used in orthopedic treatments, two major clinical challenges remained to be solved, one is the bacterial infection resulting in biofilm formation, and the other is aseptic loosening during implantation due to over-activated osteoclastogenesis. These factors can cause many clinical issues and even lead to implant failure. Thus, it is necessary to endow implants with antibiofilm and aseptic loosening-prevention properties, to facilitate the integration between implants and bone tissues for successful implantation. To achieve this goal, this study aimed to develop a biocompatible titanium alloy with antibiofilm and anti-aseptic loosening dual function by utilizing gallium (Ga) as a component. Methods A series of Ti-Ga alloys were prepared. We examined the Ga content, Ga distribution, hardness, biocompatibility, and anti-biofilm performance in vitro and in vivo. We also explored how Ga3+ ions inhibited the biofilm formation of S. aureus and E. coli and osteoclast differentiation. Results The alloy exhibited outstanding antibiofilm properties against both S. aureus and E. coli in vitro and decent antibiofilm performance against S. aureus in vivo. The proteomics results demonstrated that Ga3+ ions could disturb the bacterial Fe metabolism of both S. aureus and E. coli, inhibiting bacterial biofilm formation. In addition, Ti-Ga alloys could inhibit receptor activator of nuclear factor-κB ligand (RANKL)-dependent osteoclast differentiation and function by targeting iron metabolism, then suppressing the activation of the NF-κB signaling pathway, thus, showing their potential to prevent aseptic loosening. Conclusions This study provides an advanced Ti-Ga alloy that can be used as a promising orthopedic implant raw material for various clinical scenarios. This work also revealed that iron metabolism is the common target of Ga3+ ions to inhibit biofilm formation and osteoclast differentiation.

Publisher

Research Square Platform LLC

Reference45 articles.

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