RNA-sequence reveals differentially expressed genes affecting the secondary hyperparathyroidism

Abstract

Abstract Background/aim Secondary hyperparathyroidism (SHPT) is a common complication of chronic kidney disease (CKD), the mechanisms of cell proliferation leading to which has not been fully understood in recent years. And this study investigated the potential genes of tissue proliferation in secondary hyperparathyroidism. Materials and methods RNA sequencing (RNA-Seq) analysis was conducted to explore the potentially related genes of SHPT. Patients with secondary hyperparathyroidism indicated for surgical parathyroidectomy in our institution were reviewed and the parathyroid glands were removed for RNA-Seq analysis to measure the differential changes of RNA expression. Furthermore, we validated the differential genes expression by quantitative real-time PCR (qRT-PCR) in tissue samples and enzyme-linked immunosorbent assay (ELISA) in the serum of patients with or without SHPT. Results Our study identified 24 DEGs by RNA-Seq, among which 16 were upregulated and 8 were downregulated. Through the qRT-PCR, we further found that U2AF1L5, LTBP2, RGN in the hyperplasia group were significantly downregulated and RAP1GAP2 was significantly upregulated compared to the control group. And only Serum U2AF1L5 concentrations in patients with SHPT were significantly higher than those in healthy controls (0.360.27ng/ml vs 0.150.07ng/ml, P<0.05) as ELISA showed. Conclusion The genes including LTBP2, RGN, RAP1GAP2 and U2AF1L5 differentially expressed in tissues and serum in patients with SHPT.