The Effect of Moringa Oleifera Crude Extract on Liver Cell Line, HEPG2.

Abstract

Abstract Background The liver plays a crucial role in the body's metabolic and detoxification processes. Given its importance, compromised liver function can negatively impact the body’s metabolic and physiological function. Liver diseases can result from various factors, including exposure to toxins, alcohol consumption, and viral infections. Therefore, finding natural remedies for liver protection and treatment is of great importance. Moringa oleifera is a tree known for its various medicinal properties, including hepatoprotective effects. This study aimed to investigate the potential of Moringa oleifera seed extract in protecting liver cells. Methods In this study, dried-seed powder of Moringa oleifera was extracted using extraction solvents, methanol, and ethanol. HepG2 cells were cultured and treated with different concentrations of the extracts. Antioxidative activity, cell viability, and antiproliferation were assessed using the Total antioxidant capacity assay (TAC) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Additionally, liver enzyme activity was determined through Alkaline Phosphatase and Aspartate Aminotransferase activity assays. Results The extracts had varying effects on liver cells depending on the concentration and time of exposure. Lower concentrations (50 mg/l and 100 mg/l) appear to have mild stimulatory effects/minimal impact on metabolic activity, while higher concentrations (200 mg/l and 400 mg/l) tend to decrease metabolic activity, especially at later time points. Moreover, the extracts effectively reduced the levels of liver enzyme, AST, indicating its ability to mitigate liver injury. Conclusion The study concludes that the crude extracts of Moringa oleifera seeds exhibits potential as a natural remedy for liver diseases. The hepatoprotective effects of Moringa extract suggest that it can be used as a preventive and therapeutic agent for liver damage. The study highlights the importance of exploring natural remedies for liver protection and treatment.