Antibiotic resistance of biofilm-related catheter-associated urinary tract isolates of Pseudomonas aeruginosa

Abstract

Abstract Catheter-associated urinary tract infections (CAUTI) is one of the most common nosocomial diseases accounting for up to about 23% of healthcare-associated infections. High antibiotic resistance of biofilm-associated bacteria is a serious limiting factor in the treatment of patients. Therefore, the investigations of bacterial biofilms and their regulation can play a pivotal role in the development of new approaches to treating infections. P. aeruginosa is a key bacterium responsible for most urinary tract infections. In this study, urinary catheter-associated P. aeruginosa strains were isolated and their antibiotic resistance was studied. Polymicrobial biofilms were found on the urinary catheter surfaces derived from patients with different urinary tract diseases. Three strains of Pseudomonas aeruginosa were isolated from the catheter-associated biofilms. Their resistance to beta-lactam antibiotics (carbapenems and cephalosporins) of P. aeruginosa strains 96347 and 96349 was observed to be mediated by VIM-type metallo-β-lactamase gene expression.