Abstract
Early missed abortion is defined as a pregnancy of ≤ 12 weeks in wherein there is a cessation of life in the developing embryo or fetus, leading to its retention within the uterine cavity", failing to be expelled spontaneously in a timely manner. This is a commonly observed and significant pathological state that has an impact on the overall well-being of human reproductive health. The aim of this study was to identify key genes related to ferroptosis that could serve as novel biomarkers for early missed abortion. Relevant findings from gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicate a correlation between iron DEFRGS in key modules and the p53 signaling, mitophagy-animal, as well as protein digestion and absorption pathways. An analysis of the protein-protein interaction (PPI) network was conducted on DEFRGS, resulting in the identification of five central genes (TP53, EZH2, TIMP1, SLC3A2, and GABARAPL2) through the utilization of STRING and Cytohubba ROC curves.The expression of pivotal genes in the missed-abortion and control groups was verified by RT-qPCR. CIBERSORT analysis revealed a notable increase in the infiltration levels of CD8 T lymphocytes and M2 macrophages among individuals in the early missed abortion group. Ultimately, a ceRNA network was established in order to anticipate the connections between mRNA-miRNA-lncRNA of the central genes. However, the interacting miRNAs predicted by SLC3A2 in the miRanda, miRDB, and TargetScan databases were hsa-miR-661, hsa-miR-4311. There were no interacting lncRNAs in the spongeScan database. This research has discovered novel genes that can be targeted for the early detection and management of miscarriages.