Hydroxyapatite Based Composites for Bone Regeneration

Abstract

Abstract Aim of study: Preparation and characterization of the new nanocomposite, magneto-hydroxyapatite-alendronate (mag-HA-Ald np), for bone tissue scaffolds formation. Material and methods Iron oxide nanoparticles (Fe3O4 np) were added to aqueous solutions of Ca(NO3)2.4H2O and (NH4)2HPO4 for obtaining mag-HA np. The target nanocomposites, mag-HA-Ald np, were synthesized by using aqueous solution of alendronate (Ald), with different ratios. The structure of the prepared nanocomposites were investigated based on spectral (FTIR, XRD, SEM) and EDX analysis. The cytotoxicity of the nanocomposites were performed using MTT assay, to detect the effect of magnetic field application on the cell viability; two culture methods were applied (with and without magnetic field exposure). Results The spectral, FTIR, XRD, SEM as well as EDX measurements confirmed the synthesis of nanocomposite, magneto-hydroxyapatite-alendronate (mag-HA-Ald np) with different ratios. MTT assay results suggested the positive correlation between Ald and iron oxide content with cell viability. In addition, Magnetic field application significantly decreases the cell viability in all groups in comparison to non- magnetic field application.