Sequencing by Avidity Enables High Accuracy With Low Reagent Consumption
Author:
Arslan Sinan1, Kellinger Matthew1, Kruglyak Semyon1, LeVieux Jake1, Mah Adeline1, Wang Haosen1, Zhao Junhua1, Zhou Chunhong2, Bailey John1, Byrne Matthew1, Chang Chiting3, Chen Steve1, Dennler Claudia1, Dennler Samantha1, Dien Vivian1ORCID, Fuller Derek1, Garcia Francisco1, Guo Minghao1, Kelley Ryan1, Khandan Omid1, Klein Michael1, Kim Michael1, Lin Bill1, Liu Yu1, Lopez Tyler1, Mains Peter1, Price Andrew4, Taylor Hermes1, Tippana Ramreddy1, Tomaney Austin1, Zhang Richard1, Abtahi Minna1, Ambroso Mark1, Bajari Rosi1ORCID, Bellizi Ava1, Benitez Chris1, Berard Daniel1, Berti Lorenzo5, Blease Kelly1, Blum Angela6, Boddicker Andrew1, Bondar Leo1, Bui Chris1, Cappa Kevin7, Chan Joshua1, Chang Victor8, Charov Katia1, Chen Xiyi9, Constandse Rodger1, Damron Weston1, Dawood Mariam1, Debruno Nicole10, Dmalanta John1, Edoli Laure1, Elango Keerthi1, Faustino Nikka1, Feng Chao1, Ferrari Matthew1, Frankie Kieth1, Fries Adam1, Galloway Anne1, Gavrila Vlad1, Gemmen Gregory1, Ghadiali James1, Goddard Logan1, Roginski Adriana1, Hendricks Garren1, Hentschel Jendrick1, Hseih Doris1, Hwang-fu Yu-Hsein4, Im Scott1, Jin Chaoyi1, Kincade Daniel1, Lajooie Bryan1, Levy Shawn1, Li Yu1, Liang Vincent1, Light William1, Lipsher Jonathan1, Liu Tsungli1, Long Guixia1, Ma Rui1, Mailloux Jack1, Mandla Kyle1, Martinez Anyssa1, Mass Max1, Meron Michael1, Moh Celyne1, Moore Rachel1, Moreno Juan1, Neysmith Jordan1, Niman Cassandra1, Nunez Jesus1, Ojeda Micah1, Owens Jenna1, Ortiz Sara Espinosa1, Piland Geoffrey1, Chen Steve1, Proctor Dan1, Purba Josua1, Ray Michael1, Rong Daisong1, Saade Virginia1, Saha Sanchari1, Sirajudeen Luqmanal1, Stengel Gudrun1, Stinson Ryan1, Stone Michael1, Sundseth Keoni1, Thai Eileen1, Thompson Connor1, Tomas Gustav Santo1, Trejo Christy1, Trieger Greg1, Truong Diane1, Tse Ben1, Voiles Benjamin1, Vuong Henry1, Wong Jennifer1, Wu Chiung-Ting1, Yu Hua1, Yu Ming1, Zhang Cindy1, Zhao Da1ORCID, Zheng Frank1, He Molly1, Previte Michael1
Affiliation:
1. Element Biosciences 2. Pacific Biosciences 3. University of Washington 4. Alida Biosciences 5. Pleno Biosciences 6. Pleno 7. Singular Genomics 8. Concept Technology 9. Berkeley Lights 10. Pleno Inc
Abstract
Abstract
We present a novel sequencing chemistry implemented as part of the AVITI system. Relying on the proximal DNA binding sites created through DNA amplification on a solid support, avidity sequencing uses multivalent nucleotide ligands on dye-labeled cores to simultaneously form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets. These polymer-nucleotide substrates, termed avidites, decrease the required concentration of reporting nucleotides by 100x and yield a negligible dissociation rate. We demonstrate the use of avidites within a novel sequencing technology that surpasses Q40 accuracy and enables a diversity of applications that include single cell RNA-seq and whole human genome sequencing.
Publisher
Research Square Platform LLC
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